Composite

Part:BBa_K4257033:Design

Designed by: Ye Gu   Group: iGEM22_CPU_Nanjing   (2022-09-27)


KPD+CFPPK


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequential configuration of KPD and CFPPK in expression vector should be considered.


Source

This composite Part was commercially synthesized base on the sequences of Parts KPD and CFPPK.

References

Relyea, H.A. and Van Der Donk, W.A. (2005) Mechanism and applications of phosphite dehydrogenase. Journal of Biological Chemistry (3), 171-189.

Wang, X., Wang, X., Hui, K., Wei, W., Zhang, W., Miao, A., Xiao, L. and Yang, L. (2018) Highly effective polyphosphate synthesis, phosphate removal, and concentration using engineered environmental bacteria based on a simple solo medium-copy plasmid strategy. Environmental Science Technology 52(1), 214-222.